Parasite-Host-Cell Interactions of Eimeria papillata Sporozoites with Cultured Cells A Freeze-Fracture Study

Document Type

Article

Publication Date

1-1-1993

Keywords

Coccidia, Eimeria, Freeze-fracture, Invasion

Abstract

The relationship between the sporozoite of Eimeria papillata and the host cell was studied by comparing the frequency and distribution of intramembranous particles (IMP) in their respective plasmalemmas before and after invasion. The entering sporozoite invaginates the host cell membrane during entry and this forms the boundary of the vacuole that encloses the parasite. Freeze-fracture replicas of this parasitophorous vacuole (PV) membrane showed a 3 fold decrease in IMP on the P- face (400 ± 215) and E-face (100 ± 29) when compared to the uninfected cell membrane (1369 ± 124 on the P-face and 395 ± 79 on the E-face). Studies of other coccidia and Plasmodium suggest that this considerable modification of the membrane appears to be the result of addition of proteins and lipids which are contributed by the invading parasite. The P- and E-faces of the plasmalemma of the free or internalized sporozoite was virtually unchanged. © 1993, Gustav Fischer Verlag Jena. All rights reserved.

Journal Title

Archiv fur Protistenkunde

Volume

143

Issue

4

First Page

III

Last Page

IV

DOI

10.1016/S0003-9365(11)80334-9

First Department

Biology

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