Document Type

Article

Publication Date

1-2020

Keywords

Electrochemiluminescence, Biosensor, Dopamine, Mobile phone, Quenching

Abstract

In this work, a compact, mobile phone-based ECL sensor apparatus was developed using the phone cameras, screen-printed electrodes (SPE), and mobile app for dopamine detection. Methods of DC voltage application for ECL reaction were comprehensively studied from the mobile phone itself or external power. Under optimized sensing conditions, with disposable carbon SPE and 20 mM coreactant tri-n-propylamine (TPrA), acceptable repeatability and reproducibility were achieved in terms of relative standard deviation (RSD) of intra- and interassays, which were 6.7 and 5.5%, respectively. The biochemical compound dopamine was measured due to its ECL quenching characteristics and its clinical importance. The quenching mechanism of Ru(bpy)32+/TPrA by dopamine was investigated based on the estimation of the constants of the Stern-Volmer equations. The linear range for detectable dopamine concentration was from 1.0 to 50 μM (R2 = 0.982). As the developed mobile phone-based ECL sensor is simple, small and assembled from low-cost components, it offers new opportunities for the development of inexpensive analytical methods and compact sensors.

Journal Title

Results in Chemistry

Volume

2

Issue

100029

DOI

https://doi.org/10.1016/j.rechem.2020.100029

First Department

Engineering

Second Department

Chemistry and Biochemistry

Third Department

Population Health, Nutrition & Wellness

Acknowledgements

Retrieved 8/18/2020 from https://www.sciencedirect.com/science/article/pii/S2211715620300072?via%3Dihub#!

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