Modulation of Apoptotoic Proteins in Pancreatic Ductal Carcinoma PANC-1 Cells and Pancreatic Adenocarcinoma BxPC-3 Cells by Aqueous Extract of Chinese Medicinal Herbs Scutellaria barbata, Oldenlandia diffusa, and Bryophyllum pinnatum
Presenter Status
Professor, Department of Biology
Second Presenter Status
Student, Andrews University
Preferred Session
Poster Session
Location
Berrien Springs, MI
Start Date
20-10-2023 2:00 PM
End Date
20-10-2023 3:00 PM
Presentation Abstract
Apoptosis is a controlled program cell death process that the body uses to combat cancer. PANC-1 is a pancreatic ductal carcinoma cell while BxPC-3 is a pancreatic adenocarcinoma cell. Scutellaria barbata (SB) has been used in traditional Chinese medicine for treating liver, lung, and rectal cancers. SB and Oldenlanida diffusa (OD) are included in most of the herbal cancer treatment formulas in Taiwan hospitals. Bryophyllum pinnatum (BP) is reported to have antioxidant, immunomodulatory, anti-inflammatory, and antitumor-promoting activities. We previously reported that aqueous extracts of these three herbs selectively induced apoptosis in these two pancreatic cancer cells. In this study, we used the Human Apoptosis Antibody Array - Membrane [43 Targets] Test (Abcam) to assess the effect of these herbs in the modulation of apoptotic protein on PANC-1 and BxPC-3. Our data showed SB, OD, and BP regulate the production of various apoptosis markers such as the pro-apoptotic proteins Bad, Bax, p27, and p53 as compared to the negative controls. Modulation of some anti-apoptotic proteins like Bcl-2, IGF-I, and p21 was also observed. These results suggest that these three herbs contain phytochemicals that induce apoptosis in pancreatic PANC-1 and BxPC-3 cancer cells via the modulation of various apoptotic protein productions. Further study of their specific modulation effects of apoptosis is warranted to reveal their potential chemopreventive and therapeutic properties against pancreatic cancer.
Modulation of Apoptotoic Proteins in Pancreatic Ductal Carcinoma PANC-1 Cells and Pancreatic Adenocarcinoma BxPC-3 Cells by Aqueous Extract of Chinese Medicinal Herbs Scutellaria barbata, Oldenlandia diffusa, and Bryophyllum pinnatum
Berrien Springs, MI
Apoptosis is a controlled program cell death process that the body uses to combat cancer. PANC-1 is a pancreatic ductal carcinoma cell while BxPC-3 is a pancreatic adenocarcinoma cell. Scutellaria barbata (SB) has been used in traditional Chinese medicine for treating liver, lung, and rectal cancers. SB and Oldenlanida diffusa (OD) are included in most of the herbal cancer treatment formulas in Taiwan hospitals. Bryophyllum pinnatum (BP) is reported to have antioxidant, immunomodulatory, anti-inflammatory, and antitumor-promoting activities. We previously reported that aqueous extracts of these three herbs selectively induced apoptosis in these two pancreatic cancer cells. In this study, we used the Human Apoptosis Antibody Array - Membrane [43 Targets] Test (Abcam) to assess the effect of these herbs in the modulation of apoptotic protein on PANC-1 and BxPC-3. Our data showed SB, OD, and BP regulate the production of various apoptosis markers such as the pro-apoptotic proteins Bad, Bax, p27, and p53 as compared to the negative controls. Modulation of some anti-apoptotic proteins like Bcl-2, IGF-I, and p21 was also observed. These results suggest that these three herbs contain phytochemicals that induce apoptosis in pancreatic PANC-1 and BxPC-3 cancer cells via the modulation of various apoptotic protein productions. Further study of their specific modulation effects of apoptosis is warranted to reveal their potential chemopreventive and therapeutic properties against pancreatic cancer.