P-32 Evaluating phonotaxis variability and selective processing of its underlying neural elements in an insect model
Presenter Status
Professor, Biology
Second Presenter Status
Adjunct Professor, Biology
Third Presenter Status
Undergraduate student, School of Social and Behavioral Sciences
Fourth Presenter Status
Undergraduate students, Department of Biology
Fifth Presenter Status
Associate professor, Department of Biology
Preferred Session
Poster Session
Location
Buller Hall Hallways
Start Date
21-10-2022 2:00 PM
End Date
21-10-2022 3:00 PM
Presentation Abstract
Selective phonotaxis exhibited by female Acheta domesticus and Gryllus bimaculatus has been documented in multiple studies. While some females are finely tuned and attracted to calling songs with syllable periods matching those of the natural calls of the male, other females respond phonotactically to calls with a wider range of syllable periods. Multiple studies have also provided evidence supporting the importance of prothoracic auditory neurons such as L3/AN2 in the selective phonotaxis of female crickets. We have developed a neuronal model, which includes the contralateral omega 1 neuron inhibiting a source of delayed inhibition as well as a source of selective excitation onto the L3. The inhibition by ON1 is downregulated by Juvenile Hormone III and PTX, which increases selectivity to syllable period, and is upregulated by histamine, which leads to decreased selectivity. Testing this model has included prothoracic injections of JHIII, histamine and PTX followed by an assessment of phonotaxis and L3’s selective responses using electrophysiology. The model so far remains valid as we continue to test it. Preliminary data shows the effect of H -7 (a kinase inhibitor) on phonotaxis before and after injection parallels the effect of L3’s response. Additionally, exposure to males, decreases selectivity in females. Electrophysiology of L3’s response in exposed females is currently being evaluated.
Images
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P-32 Evaluating phonotaxis variability and selective processing of its underlying neural elements in an insect model
Buller Hall Hallways
Selective phonotaxis exhibited by female Acheta domesticus and Gryllus bimaculatus has been documented in multiple studies. While some females are finely tuned and attracted to calling songs with syllable periods matching those of the natural calls of the male, other females respond phonotactically to calls with a wider range of syllable periods. Multiple studies have also provided evidence supporting the importance of prothoracic auditory neurons such as L3/AN2 in the selective phonotaxis of female crickets. We have developed a neuronal model, which includes the contralateral omega 1 neuron inhibiting a source of delayed inhibition as well as a source of selective excitation onto the L3. The inhibition by ON1 is downregulated by Juvenile Hormone III and PTX, which increases selectivity to syllable period, and is upregulated by histamine, which leads to decreased selectivity. Testing this model has included prothoracic injections of JHIII, histamine and PTX followed by an assessment of phonotaxis and L3’s selective responses using electrophysiology. The model so far remains valid as we continue to test it. Preliminary data shows the effect of H -7 (a kinase inhibitor) on phonotaxis before and after injection parallels the effect of L3’s response. Additionally, exposure to males, decreases selectivity in females. Electrophysiology of L3’s response in exposed females is currently being evaluated.
Acknowledgments
Office of research and creative scholarship