P-31 Induction of Apoptosis in Pancreatic Ductal Carcinoma PANC-1 Cells by Chinese Medicinal Herbs Scutellaria barbata, Oldenlandia diffusa, and Brylphyllum pinnatum
Presenter Status
Biology
Second Presenter Status
Biology
Third Presenter Status
Biology
Fourth Presenter Status
Biology
Fifth Presenter Status
Biology
Sixth Presenter Status
Biology
Seventh Presenter Status
Biology
Eighth Presenter Status
Biology
Ninth Presenter Status
Biology
Preferred Session
Poster Session
Location
Buller Hall Hallways
Start Date
21-10-2022 2:00 PM
End Date
21-10-2022 3:00 PM
Presentation Abstract
Apoptosis is a controlled program cell death process that the body uses to combat cancer development. Pancreatic cancer is a devastating disease with a poor prognosis. It is usually symptom-free during development and is advanced by the time of diagnosis. PANC-1 is a pancreatic ductal carcinoma cell derived from a 56-year-old male with adenocarcinoma in the head of the pancreas and metastasized to the duodenal wall. Scutellaria barbata (SB) has been used in traditional Chinese medicine for treating liver, lung, and rectal cancers. SB and Oldenlanida diffusa (OD) are included in most of the herbal cancer treatment formulas in Taiwan hospitals. Bryophyllum pinata (BP) is reported to have antioxidant, immunomodulatory, anti-inflammatory, and antitumor-promoting activities. We previously showed that aqueous extracts of SB, OD, and BP selectively induced apoptosis in colon HCT 116 cancer cells but not in colon CCD 841 CoN normal epithelial cells by modulating the pro-apoptotic and anti-apoptotic proteins while not affecting normal colon epithelial cells. This study used the green/red/blue fluorescent Apoptosis/Necrosis Detection Kit (Abcam). Our data demonstrated that a 2-hour treatment with 1mg aqueous extract of SB, OD, and BP induced a statistically significant percentage of apoptosis in PANC-1 cells respectively. Similar results were obtained with the 4-hour incubation period. These results suggest that SB, OD, and BP contain phytochemicals that induce apoptosis in pancreatic ductal carcinoma PANC-1 cells. Further study of their specific modulation effects of apoptosis is warranted to reveal their potential chemopreventive and therapeutic properties against pancreatic cancer.
P-31 Induction of Apoptosis in Pancreatic Ductal Carcinoma PANC-1 Cells by Chinese Medicinal Herbs Scutellaria barbata, Oldenlandia diffusa, and Brylphyllum pinnatum
Buller Hall Hallways
Apoptosis is a controlled program cell death process that the body uses to combat cancer development. Pancreatic cancer is a devastating disease with a poor prognosis. It is usually symptom-free during development and is advanced by the time of diagnosis. PANC-1 is a pancreatic ductal carcinoma cell derived from a 56-year-old male with adenocarcinoma in the head of the pancreas and metastasized to the duodenal wall. Scutellaria barbata (SB) has been used in traditional Chinese medicine for treating liver, lung, and rectal cancers. SB and Oldenlanida diffusa (OD) are included in most of the herbal cancer treatment formulas in Taiwan hospitals. Bryophyllum pinata (BP) is reported to have antioxidant, immunomodulatory, anti-inflammatory, and antitumor-promoting activities. We previously showed that aqueous extracts of SB, OD, and BP selectively induced apoptosis in colon HCT 116 cancer cells but not in colon CCD 841 CoN normal epithelial cells by modulating the pro-apoptotic and anti-apoptotic proteins while not affecting normal colon epithelial cells. This study used the green/red/blue fluorescent Apoptosis/Necrosis Detection Kit (Abcam). Our data demonstrated that a 2-hour treatment with 1mg aqueous extract of SB, OD, and BP induced a statistically significant percentage of apoptosis in PANC-1 cells respectively. Similar results were obtained with the 4-hour incubation period. These results suggest that SB, OD, and BP contain phytochemicals that induce apoptosis in pancreatic ductal carcinoma PANC-1 cells. Further study of their specific modulation effects of apoptosis is warranted to reveal their potential chemopreventive and therapeutic properties against pancreatic cancer.