Presentation Title

P-31 Induction of Apoptosis in Pancreatic Ductal Carcinoma PANC-1 Cells by Chinese Medicinal Herbs Scutellaria barbata, Oldenlandia diffusa, and Brylphyllum pinnatum

Presenter Status

Biology

Second Presenter Status

Biology

Third Presenter Status

Biology

Fourth Presenter Status

Biology

Fifth Presenter Status

Biology

Sixth Presenter Status

Biology

Seventh Presenter Status

Biology

Eighth Presenter Status

Biology

Ninth Presenter Status

Biology

Preferred Session

Poster Session

Location

Buller Hall Hallways

Start Date

21-10-2022 2:00 PM

End Date

21-10-2022 3:00 PM

Presentation Abstract

Apoptosis is a controlled program cell death process that the body uses to combat cancer development. Pancreatic cancer is a devastating disease with a poor prognosis. It is usually symptom-free during development and is advanced by the time of diagnosis. PANC-1 is a pancreatic ductal carcinoma cell derived from a 56-year-old male with adenocarcinoma in the head of the pancreas and metastasized to the duodenal wall. Scutellaria barbata (SB) has been used in traditional Chinese medicine for treating liver, lung, and rectal cancers. SB and Oldenlanida diffusa (OD) are included in most of the herbal cancer treatment formulas in Taiwan hospitals. Bryophyllum pinata (BP) is reported to have antioxidant, immunomodulatory, anti-inflammatory, and antitumor-promoting activities. We previously showed that aqueous extracts of SB, OD, and BP selectively induced apoptosis in colon HCT 116 cancer cells but not in colon CCD 841 CoN normal epithelial cells by modulating the pro-apoptotic and anti-apoptotic proteins while not affecting normal colon epithelial cells. This study used the green/red/blue fluorescent Apoptosis/Necrosis Detection Kit (Abcam). Our data demonstrated that a 2-hour treatment with 1mg aqueous extract of SB, OD, and BP induced a statistically significant percentage of apoptosis in PANC-1 cells respectively. Similar results were obtained with the 4-hour incubation period. These results suggest that SB, OD, and BP contain phytochemicals that induce apoptosis in pancreatic ductal carcinoma PANC-1 cells. Further study of their specific modulation effects of apoptosis is warranted to reveal their potential chemopreventive and therapeutic properties against pancreatic cancer.

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Oct 21st, 2:00 PM Oct 21st, 3:00 PM

P-31 Induction of Apoptosis in Pancreatic Ductal Carcinoma PANC-1 Cells by Chinese Medicinal Herbs Scutellaria barbata, Oldenlandia diffusa, and Brylphyllum pinnatum

Buller Hall Hallways

Apoptosis is a controlled program cell death process that the body uses to combat cancer development. Pancreatic cancer is a devastating disease with a poor prognosis. It is usually symptom-free during development and is advanced by the time of diagnosis. PANC-1 is a pancreatic ductal carcinoma cell derived from a 56-year-old male with adenocarcinoma in the head of the pancreas and metastasized to the duodenal wall. Scutellaria barbata (SB) has been used in traditional Chinese medicine for treating liver, lung, and rectal cancers. SB and Oldenlanida diffusa (OD) are included in most of the herbal cancer treatment formulas in Taiwan hospitals. Bryophyllum pinata (BP) is reported to have antioxidant, immunomodulatory, anti-inflammatory, and antitumor-promoting activities. We previously showed that aqueous extracts of SB, OD, and BP selectively induced apoptosis in colon HCT 116 cancer cells but not in colon CCD 841 CoN normal epithelial cells by modulating the pro-apoptotic and anti-apoptotic proteins while not affecting normal colon epithelial cells. This study used the green/red/blue fluorescent Apoptosis/Necrosis Detection Kit (Abcam). Our data demonstrated that a 2-hour treatment with 1mg aqueous extract of SB, OD, and BP induced a statistically significant percentage of apoptosis in PANC-1 cells respectively. Similar results were obtained with the 4-hour incubation period. These results suggest that SB, OD, and BP contain phytochemicals that induce apoptosis in pancreatic ductal carcinoma PANC-1 cells. Further study of their specific modulation effects of apoptosis is warranted to reveal their potential chemopreventive and therapeutic properties against pancreatic cancer.