Presenter Status
Graduate Student Researcher, Biological Sciences
Presentation Type
Oral Presentation
Session
C
Location
Chan Shun 108
Start Date
19-5-2017 10:55 AM
End Date
19-5-2017 11:15 AM
Presentation Abstract
Streptococcus mutans JH1140 is a strain of bacteria which produces a lantibiotic product, named mutacin 1140. Mutacin 1140 has been shown to be effective at inhibiting Gram-positive bacterial infections caused by Staphylococcus aureus and Streptococcus pneumoniae. Mutacin 1140 is a ribosomally synthesized peptide antibiotic that undergoes extensive posttranslational modifications (PTM). We have found that Mutacin 1140 and an aminoglycoside, Kanamycin, when combined together, act synergistically against Staphylococcus aureus. This was determined by performing serial kill curve dilution overlays on solid media, followed up with kill curve by microdilution plate, and most recently confirmed with kill curve CFU count plates on Thyex media over time points in a 24 hour period. All three methods are independently consistent with their results: in combination with Kanamycin, Mutacin 1140 kills at an improved rate than either compound individually. Synergistic behavior opens many interesting opportunities for altered treatment strategies (lowered doses necessary for efficacy) and decreases the risk of developed resistance by Staphylococcus aureus against either 1140 or Kanamycin by providing multiple effective drug targets to account for.
Biographical Sketch
Steven Lai Hing was born and raised in Huntsville Alabama. He attended Oakwood University (Huntsville, AL) where he received a Bachelors degree in biochemistry. Following his time at Oakwood he accepted a research fellowship to Texas A&M University (College Station,TX) where he completed a Masters in Biochemistry, and where he is currently writing his dissertation for a Doctorate in Microbiology. His research interests are in antibiotic design, drug combination therapies, and natural products. He also greatly enjoys teaching.
Files over 3MB may be slow to open. For best results, right-click and select "save as..."
Included in
Cell Biology Commons, Molecular Biology Commons, Natural Products Chemistry and Pharmacognosy Commons, Pharmacology Commons
Applied Drug Development and Combinatorial Strategies for Antimicrobial Treatment
Chan Shun 108
Streptococcus mutans JH1140 is a strain of bacteria which produces a lantibiotic product, named mutacin 1140. Mutacin 1140 has been shown to be effective at inhibiting Gram-positive bacterial infections caused by Staphylococcus aureus and Streptococcus pneumoniae. Mutacin 1140 is a ribosomally synthesized peptide antibiotic that undergoes extensive posttranslational modifications (PTM). We have found that Mutacin 1140 and an aminoglycoside, Kanamycin, when combined together, act synergistically against Staphylococcus aureus. This was determined by performing serial kill curve dilution overlays on solid media, followed up with kill curve by microdilution plate, and most recently confirmed with kill curve CFU count plates on Thyex media over time points in a 24 hour period. All three methods are independently consistent with their results: in combination with Kanamycin, Mutacin 1140 kills at an improved rate than either compound individually. Synergistic behavior opens many interesting opportunities for altered treatment strategies (lowered doses necessary for efficacy) and decreases the risk of developed resistance by Staphylococcus aureus against either 1140 or Kanamycin by providing multiple effective drug targets to account for.
Acknowledgements
Dr. James Smith (PI)
Texas A&M Department of Biological Sciences
TAMU Lower Division Biology for funding (Teaching Assistantship)