Date of Award
Master of Science
College of Arts and Sciences
The thesis objective was to determine if myo-inositol concentration levels and the gene expression of IMPA2 & ISYNA1 differ among bipolar disorder types 1 and 2 compared to healthy controls (non-bipolar disorder). Previous studies have correlated different myo-inositol concentration levels with specific phases of bipolar and pharmaceutical treatments. Two genes, inositol monophosphatase 2 (IMPA2) and inositol-3-phosphate synthase 1 (ISYNA1), involved in de novo myo-inositol biosynthesis have been implicated in the pathophysiology of bipolar. In this study, differences in myo-inositol concentration and IMPA2 & ISYNA1 gene expression in lymphoblasts derived from subjects with bipolar types 1, 2, and healthy control were measured spectrophotometrically and by RT-qPCR respectively. Myo-inositol concentration assay results showed statistically significant differences in myo-inositol concentration in bipolar type 1 compared to type 2 and non-bipolar disorder. The relative gene expression of IMPA2 was twofold higher in both types of bipolar compared to healthy controls. The relative gene expression of ISYNA1 was 0.47-fold higher in bipolar 1; and 0.76-fold higher in bipolar 2 compared to healthy control. This study found both ISYNA1 and IMPA2 expressed relatively higher in bipolar compared to non-bipolar; and bipolar type 1 had significantly higher concentrations of myo-inositol compared to type 2 and non-bipolar disorder.
Manic-depressive illness; Inositol; Inositol phosphates
Rosette, Christina, "A Comparison of IMPA2 & ISYNA1 Gene Expression and Intracelular Myo-Inositol Levels in Bipolar and Non-bipolar Disorder Derived Human Lymphoblasts" (2022). Master's Theses. 200.
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