Date of Award

2022

Document Type

Thesis

Degree Name

Master of Science

College

College of Arts and Sciences

Program

Biology, MS

First Advisor

Marlene Murray

Abstract

The thesis objective was to determine if myo-inositol concentration levels and the gene expression of IMPA2 & ISYNA1 differ among bipolar disorder types 1 and 2 compared to healthy controls (non-bipolar disorder). Previous studies have correlated different myo-inositol concentration levels with specific phases of bipolar and pharmaceutical treatments. Two genes, inositol monophosphatase 2 (IMPA2) and inositol-3-phosphate synthase 1 (ISYNA1), involved in de novo myo-inositol biosynthesis have been implicated in the pathophysiology of bipolar. In this study, differences in myo-inositol concentration and IMPA2 & ISYNA1 gene expression in lymphoblasts derived from subjects with bipolar types 1, 2, and healthy control were measured spectrophotometrically and by RT-qPCR respectively. Myo-inositol concentration assay results showed statistically significant differences in myo-inositol concentration in bipolar type 1 compared to type 2 and non-bipolar disorder. The relative gene expression of IMPA2 was twofold higher in both types of bipolar compared to healthy controls. The relative gene expression of ISYNA1 was 0.47-fold higher in bipolar 1; and 0.76-fold higher in bipolar 2 compared to healthy control. This study found both ISYNA1 and IMPA2 expressed relatively higher in bipolar compared to non-bipolar; and bipolar type 1 had significantly higher concentrations of myo-inositol compared to type 2 and non-bipolar disorder.

Subject Area

Manic-depressive illness; Inositol; Inositol phosphates

DOI

https://dx.doi.org/10.32597/theses/200/

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