Date of Award
4-5-2021
Document Type
Honors Thesis
Department
Chemistry & Biochemistry
First Advisor
Lisa Ahlberg
Abstract
Three-dimensional fluorometric scans of OPA-IBLC derivatized glutamic acid and histidine solutions revealed that decreasing concentration maintained maximum λem but produced different maximum λex. In pH 0.4 M sodium borate buffer (pH 10.4), 50 μM derivatized amino acid solutions had maxima around 350- to 450-nm λex-λem where 12.5 μM solutions had maxima around 230- to 450-nm λex- λem. In the lower concentration, the λex peak at 230 nm was about 80% higher than 350 nm. Thus, collecting spectra with 230 nm λex would provide better LOD and LOQ. Results inform HPLC standard concentrations since differential quenching effects may produce non-linear calibration curves.
Recommended Citation
Jacobs, Aaron, "Finding Optimal OPA-IBLC Derivatized Amino Acid Analysis Parameters with Three-Dimensional Fluorometric Scans for HPLC Method Validation" (2021). Honors Theses. 249.
https://dx.doi.org/10.32597/honors/249/
https://digitalcommons.andrews.edu/honors/249
Subject Area
High performance liquid chromatography; Amino acids
Presentation Record URL
https://digitalcommons.andrews.edu/honors-undergraduate-poster-symposium/2021/symposium/7/
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
DOI
https://dx.doi.org/10.32597/honors/249/