Date of Award
4-30-2020
Document Type
Honors Thesis
Department
Chemistry & Biochemistry
First Advisor
Lisa Ahlberg
Abstract
The extent of amino acid racemization (AAR) observed in organic material offers insight into geochronology. Within the scope of AAR research, Avian eggshells are reliable sources of data due to retention of indigenous amino acids through calcification. This project develops a robust quantification methodology for up to 9 D/L amino acids using reverse-phase HPLC (RP-HPLC) with precolumn derivatization. Reliable quantitation is established via statistical assessment of detectability and calibration curves. Progress has focused on obtaining adequate enantioseparation and reproducible data of single D/L amino acids.
Recommended Citation
Nelapudi, Nikitha, "Assessing Amino Acid Racemization in Avian Eggshells through Reverse Phase Liquid Chromatography" (2020). Honors Theses. 234.
https://dx.doi.org/10.32597/honors/234/
https://digitalcommons.andrews.edu/honors/234
Subject Area
Amino acids; Racemization; Eggshells; Birds--Eggs; Chromatographic analysis; Hydrophilic interaction liquid chromatography
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
DOI
https://dx.doi.org/10.32597/honors/234/