Prep-HPLC Method Development to Isolate Potentially-Carcinogenic, Arginine-Based Heterocyclic Amines
Date of Award
12-6-2017
Document Type
Honors Thesis
Department
Chemistry & Biochemistry
First Advisor
Ryan T. Hayes
Abstract
Heterocyclic amines (HCAs) are carcinogenic molecules generated from the reaction of creatin(in)e and amino acids at high cooking temperatures in meat. Previous research has shown that replacing creatine with arginine leads to new, uncharacterized HCA molecules. This research entails implementing and optimizing the preparative HPLC analysis of extracts from multiple burnings of arginine with phenylalanine. Isolated fractions from the prep-HPLC analysis were further analyzed using the Ames test to identify mutagenic compounds. Prep-HPLC provides quality control to the burning process along with isolation of larger quantities of materials for further characterization and molecular structure identification.
Recommended Citation
Kim, Victoria S., "Prep-HPLC Method Development to Isolate Potentially-Carcinogenic, Arginine-Based Heterocyclic Amines" (2017). Honors Theses. 177.
https://dx.doi.org/10.32597/honors/177/
https://digitalcommons.andrews.edu/honors/177
Subject Area
Heterocyclic amines; Creatinine; Arginine; Mutagens
Creative Commons License
This work is licensed under a Creative Commons Attribution-No Derivative Works 4.0 International License.
DOI
https://dx.doi.org/10.32597/honors/177/
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