P-20 Analysis of Invasion Proteins in Glioblastoma U87MG Cells Treated with Various Novel Hybrid Anti-cancer Compounds

Department

Biology

Abstract

This research tested effectiveness of compounds 2-TBH, PKP2, PKP3, PKP5, and DMOB, demonstrated to have anti-cancer properties. Glioblastoma cells were exposed to each compound at the LC50 concentration, then processed to collect proteins from the cells. Proteins were analyzed via Western blotting for specific protein levels of matrix metalloproteinase 2 and 9 (MMP) and disintegrin and metalloprotease 12 and 17 (ADAM). Previous research indicates these proteins are involved in the invasive properties of glioblastoma cells. Preliminary results show that the different compounds result in varying levels of MMP-2 and 9 as well as ADAM-12 and 17 proteins.

Acknowledgments

Advisor: Denise Smith, Biology

Location

Buller Hall 250

Start Date

3-11-2022 1:30 PM

End Date

3-11-2022 3:30 PM

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Mar 11th, 1:30 PM Mar 11th, 3:30 PM

P-20 Analysis of Invasion Proteins in Glioblastoma U87MG Cells Treated with Various Novel Hybrid Anti-cancer Compounds

Buller Hall 250

This research tested effectiveness of compounds 2-TBH, PKP2, PKP3, PKP5, and DMOB, demonstrated to have anti-cancer properties. Glioblastoma cells were exposed to each compound at the LC50 concentration, then processed to collect proteins from the cells. Proteins were analyzed via Western blotting for specific protein levels of matrix metalloproteinase 2 and 9 (MMP) and disintegrin and metalloprotease 12 and 17 (ADAM). Previous research indicates these proteins are involved in the invasive properties of glioblastoma cells. Preliminary results show that the different compounds result in varying levels of MMP-2 and 9 as well as ADAM-12 and 17 proteins.