P-12 Optimization of an Integrated Cartridge System for Efficient Loading and Characterization of DNA through Microfluidic Platforms

Department

Chemistry and Biochemistry

Abstract

Microfluidic platforms have critical implications for field-based clinical diagnostics. They can aid in accelerating the process of diagnosis, which is crucial for patients who are too ill to wait several days for results. The objective of this project was to create a cartridge system that improves the efficiency of loading and running biochemical assays in microfluidic platforms. This was accomplished by designing three separate cartridges made of polydimethylsiloxane (PDMS), laser-cut acrylic, and 3D-printed resin, and subsequently evaluating their ability to load a microfluidic chip once pressurized. Ultimately, 3D-printed cartridges were shown to be the most effective in loading microfluidic platforms.

Location

Buller Hall 149

Start Date

3-11-2022 1:30 PM

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Mar 11th, 1:30 PM

P-12 Optimization of an Integrated Cartridge System for Efficient Loading and Characterization of DNA through Microfluidic Platforms

Buller Hall 149

Microfluidic platforms have critical implications for field-based clinical diagnostics. They can aid in accelerating the process of diagnosis, which is crucial for patients who are too ill to wait several days for results. The objective of this project was to create a cartridge system that improves the efficiency of loading and running biochemical assays in microfluidic platforms. This was accomplished by designing three separate cartridges made of polydimethylsiloxane (PDMS), laser-cut acrylic, and 3D-printed resin, and subsequently evaluating their ability to load a microfluidic chip once pressurized. Ultimately, 3D-printed cartridges were shown to be the most effective in loading microfluidic platforms.