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Department

Biology

Abstract

Scutellaria barbata (SB), a Chinese medical herb, has been known to contain anti-cancer properties. In this study, the effectiveness of SB in apoptotic modulation of APC-mutant breast cancer cell lines MDA-MB-157, 93A, and 93B were investigated. Assessments were performed using the green/red/blue fluorescent Apoptosis/Necrosis Detection Kit and the Human Apoptosis Antibody Array -Membrane (43 Targets) test by
the Abcam cooperation. Our data demonstrated that 1-hour and 3-hour incubation treatments with aqueous extract of SB induced apoptosis in all cell lines. Additionally, modulation of pro-apoptotic markers (Bad,
Bax, BID, BIM, C3, C8, p53, p27), and anti-apoptotic markers (BcL-2, Bcl-w, p21) were observed.

Acknowledgments

Advisor: Brian Wong

Thesis Record URL

https://digitalcommons.andrews.edu/honors/253/

Session

Department of Biology

Event Website

https://www.andrews.edu/services/research/research_events/conferences/urs_honors_poster_symposium/index.html

Start Date

3-26-2021 2:20 PM

End Date

3-26-2021 2:40 PM

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COinS
 
Mar 26th, 2:20 PM Mar 26th, 2:40 PM

Modulation of Apoptosis in Breast Cancer Cells MDA-MB-157, 93A and 93B by Aqueous Extract of Chinese Medical Herb Scutel

Scutellaria barbata (SB), a Chinese medical herb, has been known to contain anti-cancer properties. In this study, the effectiveness of SB in apoptotic modulation of APC-mutant breast cancer cell lines MDA-MB-157, 93A, and 93B were investigated. Assessments were performed using the green/red/blue fluorescent Apoptosis/Necrosis Detection Kit and the Human Apoptosis Antibody Array -Membrane (43 Targets) test by
the Abcam cooperation. Our data demonstrated that 1-hour and 3-hour incubation treatments with aqueous extract of SB induced apoptosis in all cell lines. Additionally, modulation of pro-apoptotic markers (Bad,
Bax, BID, BIM, C3, C8, p53, p27), and anti-apoptotic markers (BcL-2, Bcl-w, p21) were observed.

https://digitalcommons.andrews.edu/honors-undergraduate-poster-symposium/2021/symposium/5