P-41 The Role of the Prodomain in Folding of Carboxypeptidases
Abstract
Carboxypeptidase A1 (CPA1) is a digestive enzyme produced by the pancreas. Previous research has suggested that one part of CPA1, the prodomain, is necessary for the rest of the carboxypeptidase to assume proper form and function enzymatically. However, a related carboxypeptidase, CPO, does not require a prodomain. In order to confirm the need for a prodomain in CPA1, the protein was tagged using recombinant DNA techniques with an HA-tag for detection. HEK293T cells were transfected and the enzymatic activity of these tagged proteins was measured. Future work will involve removal of the prodomain for comparison.
Start Date
2-28-2020 2:30 PM
P-41 The Role of the Prodomain in Folding of Carboxypeptidases
Carboxypeptidase A1 (CPA1) is a digestive enzyme produced by the pancreas. Previous research has suggested that one part of CPA1, the prodomain, is necessary for the rest of the carboxypeptidase to assume proper form and function enzymatically. However, a related carboxypeptidase, CPO, does not require a prodomain. In order to confirm the need for a prodomain in CPA1, the protein was tagged using recombinant DNA techniques with an HA-tag for detection. HEK293T cells were transfected and the enzymatic activity of these tagged proteins was measured. Future work will involve removal of the prodomain for comparison.
Acknowledgments
J.N. Andrews Honors Scholar and Undergraduate Research Scholar
Mentor: Peter Lyons, Biology