Poster Title
P-42 Method Optimization to Isolate Potentially-Carcinogenic, Arginine-Based Heterocyclic Amines
Abstract
Burning creatinine and phenylalanine together produces, in very small quantities, PhIP, which is a known HeteroCyclicAmine(HCA)-type carcinogen. PhIP can be purchased and used a control to determine the effectiveness of burning, extracting, and purifying HCAs from burnt amino acids. This study evaluated the steps to create, extract, and analyze the production of PhIP from burning creatinine with phenylalanine. This process can be used to train students and make improvements to existing protocols and procedures, such as how to best utilize and recycle expensive PRS cartridges that are used in the isolation of HCA.
Location
Buller Hall Lobby
Start Date
3-8-2019 2:30 PM
P-42 Method Optimization to Isolate Potentially-Carcinogenic, Arginine-Based Heterocyclic Amines
Buller Hall Lobby
Burning creatinine and phenylalanine together produces, in very small quantities, PhIP, which is a known HeteroCyclicAmine(HCA)-type carcinogen. PhIP can be purchased and used a control to determine the effectiveness of burning, extracting, and purifying HCAs from burnt amino acids. This study evaluated the steps to create, extract, and analyze the production of PhIP from burning creatinine with phenylalanine. This process can be used to train students and make improvements to existing protocols and procedures, such as how to best utilize and recycle expensive PRS cartridges that are used in the isolation of HCA.
Acknowledgments
Supervising Professor: Ryan T. Hayes