Event Title

P-17 Analysis of DL-Amino Acids Ratios in Eggshells using Reverse Phase-High Pressure Liquid Chromatography

Presenter Information

Gergana Milkova, Andrews University

Location

Buller Hall Lobby

Start Date

3-8-2019 2:30 PM

Description

HPLC methodology was developed to determine the concentrations and ratios of D to L amino acids in emu and ostrich eggshells treated with heat at different temperatures. We aim to determine an internal standard and how ratios were affected under different conditions. An HPLC method was determined that gave consistent retention times and satisfactory enantioseparation. Calibration curves for each amino acid were developed from single and multi-amino acid containing dilution series producing a model that most closely replicates the eggshell-extracted amino acids.

Acknowledgments

Supervising Professor: Dr. Lisa Ahlberg

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Mar 8th, 2:30 PM

P-17 Analysis of DL-Amino Acids Ratios in Eggshells using Reverse Phase-High Pressure Liquid Chromatography

Buller Hall Lobby

HPLC methodology was developed to determine the concentrations and ratios of D to L amino acids in emu and ostrich eggshells treated with heat at different temperatures. We aim to determine an internal standard and how ratios were affected under different conditions. An HPLC method was determined that gave consistent retention times and satisfactory enantioseparation. Calibration curves for each amino acid were developed from single and multi-amino acid containing dilution series producing a model that most closely replicates the eggshell-extracted amino acids.