Poster Title
P-17 Analysis of DL-Amino Acids Ratios in Eggshells using Reverse Phase-High Pressure Liquid Chromatography
Abstract
HPLC methodology was developed to determine the concentrations and ratios of D to L amino acids in emu and ostrich eggshells treated with heat at different temperatures. We aim to determine an internal standard and how ratios were affected under different conditions. An HPLC method was determined that gave consistent retention times and satisfactory enantioseparation. Calibration curves for each amino acid were developed from single and multi-amino acid containing dilution series producing a model that most closely replicates the eggshell-extracted amino acids.
Location
Buller Hall Lobby
Start Date
3-8-2019 2:30 PM
P-17 Analysis of DL-Amino Acids Ratios in Eggshells using Reverse Phase-High Pressure Liquid Chromatography
Buller Hall Lobby
HPLC methodology was developed to determine the concentrations and ratios of D to L amino acids in emu and ostrich eggshells treated with heat at different temperatures. We aim to determine an internal standard and how ratios were affected under different conditions. An HPLC method was determined that gave consistent retention times and satisfactory enantioseparation. Calibration curves for each amino acid were developed from single and multi-amino acid containing dilution series producing a model that most closely replicates the eggshell-extracted amino acids.
Acknowledgments
Supervising Professor: Dr. Lisa Ahlberg