Event Title

P-05 HPLC Analysis of Amino Acids in Glaucous-winged Gull Eggshells

Start Date

3-2-2018 2:30 PM

Description

We are developing a reliable method of analyzing amino acid contents of eggshells using High Pressure Liquid Chromatography (HPLC). The closed-system nature of eggshells allows for accurate analysis. The method of analysis requires dissolution, derivatization, and an HPLC running method which includes a column cleaning routine. The amino acids are derivatized with o-phthaldialdehyde (OPA) and N-isobutyryl- L-cysteine (IBLC) to add fluorescence capability and improve D- and L-isomer separation. The derivatized amino acid solution is injected into the HPLC with a low pH buffer using methanol/water, and then analyzed with fluorescence spectroscopy. This quantification method can be used for further analysis, like racemization dating.

Acknowledgments

Dr. Lisa Ahlberg.

J. N. Andrews Honors Program.

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Mar 2nd, 2:30 PM

P-05 HPLC Analysis of Amino Acids in Glaucous-winged Gull Eggshells

We are developing a reliable method of analyzing amino acid contents of eggshells using High Pressure Liquid Chromatography (HPLC). The closed-system nature of eggshells allows for accurate analysis. The method of analysis requires dissolution, derivatization, and an HPLC running method which includes a column cleaning routine. The amino acids are derivatized with o-phthaldialdehyde (OPA) and N-isobutyryl- L-cysteine (IBLC) to add fluorescence capability and improve D- and L-isomer separation. The derivatized amino acid solution is injected into the HPLC with a low pH buffer using methanol/water, and then analyzed with fluorescence spectroscopy. This quantification method can be used for further analysis, like racemization dating.