P-05 HPLC Analysis of Amino Acids in Glaucous-winged Gull Eggshells
Abstract
We are developing a reliable method of analyzing amino acid contents of eggshells using High Pressure Liquid Chromatography (HPLC). The closed-system nature of eggshells allows for accurate analysis. The method of analysis requires dissolution, derivatization, and an HPLC running method which includes a column cleaning routine. The amino acids are derivatized with o-phthaldialdehyde (OPA) and N-isobutyryl- L-cysteine (IBLC) to add fluorescence capability and improve D- and L-isomer separation. The derivatized amino acid solution is injected into the HPLC with a low pH buffer using methanol/water, and then analyzed with fluorescence spectroscopy. This quantification method can be used for further analysis, like racemization dating.
Start Date
3-2-2018 2:30 PM
P-05 HPLC Analysis of Amino Acids in Glaucous-winged Gull Eggshells
We are developing a reliable method of analyzing amino acid contents of eggshells using High Pressure Liquid Chromatography (HPLC). The closed-system nature of eggshells allows for accurate analysis. The method of analysis requires dissolution, derivatization, and an HPLC running method which includes a column cleaning routine. The amino acids are derivatized with o-phthaldialdehyde (OPA) and N-isobutyryl- L-cysteine (IBLC) to add fluorescence capability and improve D- and L-isomer separation. The derivatized amino acid solution is injected into the HPLC with a low pH buffer using methanol/water, and then analyzed with fluorescence spectroscopy. This quantification method can be used for further analysis, like racemization dating.
Acknowledgments
Dr. Lisa Ahlberg.
J. N. Andrews Honors Program.