P-01 Mutagenicity (Ames) Test Optimization for Evaluating Mutagenicity of Burned Arginine-based Heterocyclic Amines and the Anti-mutagenic Effect of Chinese Medicinal Herbs
Abstract
From the unexpected finding that cooked grains and meat substitutes elicit a mutagenic response in Salmonella typhimurium TA98, our work has been aimed at deconstructing this finding via a survey of heated binary amino acid combinations, involving arginine, a plant-based amino acid. Secondly, our work has looked for possible substances, such as traditional Chinese medicinal herbs, that can inhibit the mutagenic activity of heterocyclic amines (HCAs, the main culprit for mutagenicity in meat). Our findings indicate that mutagenic activity exists with HCA-like products from heated amino acids and that the tested Chinese herbs effectively inhibit a known HCA, PhIP.
Thesis Record URL
https://digitalcommons.andrews.edu/honors/172
Start Date
3-2-2018 2:30 PM
P-01 Mutagenicity (Ames) Test Optimization for Evaluating Mutagenicity of Burned Arginine-based Heterocyclic Amines and the Anti-mutagenic Effect of Chinese Medicinal Herbs
From the unexpected finding that cooked grains and meat substitutes elicit a mutagenic response in Salmonella typhimurium TA98, our work has been aimed at deconstructing this finding via a survey of heated binary amino acid combinations, involving arginine, a plant-based amino acid. Secondly, our work has looked for possible substances, such as traditional Chinese medicinal herbs, that can inhibit the mutagenic activity of heterocyclic amines (HCAs, the main culprit for mutagenicity in meat). Our findings indicate that mutagenic activity exists with HCA-like products from heated amino acids and that the tested Chinese herbs effectively inhibit a known HCA, PhIP.
Acknowledgments
Dr. Ryan T. Hayes, Dr. Brian Y. Y. Wong.
J. N. Andrews Honors Program.
AU Office of Research & Creative Scholarship.