Presentation Title

P-18 Chinese Medicinal herb Scutellaria barbata Modulates Apoptosis via Induction of Pro-apoptotic and Anti-apoptotic Proteins in MDA-MB-157, 93A, and 93B Breast Cancer Cells

Presenter Status

Professor, Department of Biology

Second Presenter Status

Student

Third Presenter Status

Student

Fourth Presenter Status

Student

Fifth Presenter Status

Student

Sixth Presenter Status

Student

Seventh Presenter Status

Student

Eighth Presenter Status

Student

Ninth Presenter Status

Student

Tenth Presenter Status

Student

Preferred Session

Poster Session

Location

Buller Hall Hallways

Start Date

22-10-2021 2:00 PM

End Date

22-10-2021 2:00 PM

Presentation Abstract

Scutellaria barbata (SB has been used in traditional Chinese medicine for treating liver, lung, and rectal cancers. It is included in most of the herbal cancer treatment formulas in Taiwan hospitals. We previously showed that aqueous extracts of SB inhibited mutagenesis, DNA binding, and metabolism of aflatoxin B1 and benzo(apyrene in carcinogenesis. A fraction of it was studied in a preliminary clinical trial in breast cancer patients. Breast cancer cell lines MDA-MB-157, 93A, and 93B are drug-resistant APC-mutants. In this study, the effectiveness of SB in the modulation of apoptosis of breast cancer cell lines MDA-MB-157, 93A, and 93B was investigated. Assessments were performed using green/red/blue fluorescent Apoptosis/Necrosis Detection Kit and the Human Apoptosis Antibody Array - Membrane (43 Targets test by the Abcam cooperation. Our data demonstrated that 1-hour treatment with 2 mg aqueous extract of SB induced a statistically significant percentage of apoptosis in MDA-MB- 157 (46.5 ± 7.5% > 16%± 1.0% , p < 0.05 ; 93A (54.5 ± 2.5% > 0%, p < 0.05; and 93 B (65.5 ± 13.5% > 0%, p < 0.05. Similar results were obtained with the 3-hour incubation period. Modulation of various apoptosis markers such as pro-apoptotic Bad, Bax, p53; and anti-apoptotic markers BcL-2, p21, and FasL also observed. These results suggest that SB contains phytochemicals that induce apoptosis in MDA-MB-157, 93A, and 93B by modulating these pro-apoptotic and anti-apoptotic proteins.

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COinS
 
Oct 22nd, 2:00 PM Oct 22nd, 2:00 PM

P-18 Chinese Medicinal herb Scutellaria barbata Modulates Apoptosis via Induction of Pro-apoptotic and Anti-apoptotic Proteins in MDA-MB-157, 93A, and 93B Breast Cancer Cells

Buller Hall Hallways

Scutellaria barbata (SB has been used in traditional Chinese medicine for treating liver, lung, and rectal cancers. It is included in most of the herbal cancer treatment formulas in Taiwan hospitals. We previously showed that aqueous extracts of SB inhibited mutagenesis, DNA binding, and metabolism of aflatoxin B1 and benzo(apyrene in carcinogenesis. A fraction of it was studied in a preliminary clinical trial in breast cancer patients. Breast cancer cell lines MDA-MB-157, 93A, and 93B are drug-resistant APC-mutants. In this study, the effectiveness of SB in the modulation of apoptosis of breast cancer cell lines MDA-MB-157, 93A, and 93B was investigated. Assessments were performed using green/red/blue fluorescent Apoptosis/Necrosis Detection Kit and the Human Apoptosis Antibody Array - Membrane (43 Targets test by the Abcam cooperation. Our data demonstrated that 1-hour treatment with 2 mg aqueous extract of SB induced a statistically significant percentage of apoptosis in MDA-MB- 157 (46.5 ± 7.5% > 16%± 1.0% , p < 0.05 ; 93A (54.5 ± 2.5% > 0%, p < 0.05; and 93 B (65.5 ± 13.5% > 0%, p < 0.05. Similar results were obtained with the 3-hour incubation period. Modulation of various apoptosis markers such as pro-apoptotic Bad, Bax, p53; and anti-apoptotic markers BcL-2, p21, and FasL also observed. These results suggest that SB contains phytochemicals that induce apoptosis in MDA-MB-157, 93A, and 93B by modulating these pro-apoptotic and anti-apoptotic proteins.