P-18 Chinese Medicinal herb Scutellaria barbata Modulates Apoptosis via Induction of Pro-apoptotic and Anti-apoptotic Proteins in MDA-MB-157, 93A, and 93B Breast Cancer Cells
Presenter Status
Professor, Department of Biology
Second Presenter Status
Student
Third Presenter Status
Student
Fourth Presenter Status
Student
Fifth Presenter Status
Student
Sixth Presenter Status
Student
Seventh Presenter Status
Student
Eighth Presenter Status
Student
Ninth Presenter Status
Student
Tenth Presenter Status
Student
Preferred Session
Poster Session
Location
Buller Hall Hallways
Start Date
22-10-2021 2:00 PM
End Date
22-10-2021 2:00 PM
Presentation Abstract
Scutellaria barbata (SB has been used in traditional Chinese medicine for treating liver, lung, and rectal cancers. It is included in most of the herbal cancer treatment formulas in Taiwan hospitals. We previously showed that aqueous extracts of SB inhibited mutagenesis, DNA binding, and metabolism of aflatoxin B1 and benzo(apyrene in carcinogenesis. A fraction of it was studied in a preliminary clinical trial in breast cancer patients. Breast cancer cell lines MDA-MB-157, 93A, and 93B are drug-resistant APC-mutants. In this study, the effectiveness of SB in the modulation of apoptosis of breast cancer cell lines MDA-MB-157, 93A, and 93B was investigated. Assessments were performed using green/red/blue fluorescent Apoptosis/Necrosis Detection Kit and the Human Apoptosis Antibody Array - Membrane (43 Targets test by the Abcam cooperation. Our data demonstrated that 1-hour treatment with 2 mg aqueous extract of SB induced a statistically significant percentage of apoptosis in MDA-MB- 157 (46.5 ± 7.5% > 16%± 1.0% , p < 0.05 ; 93A (54.5 ± 2.5% > 0%, p < 0.05; and 93 B (65.5 ± 13.5% > 0%, p < 0.05. Similar results were obtained with the 3-hour incubation period. Modulation of various apoptosis markers such as pro-apoptotic Bad, Bax, p53; and anti-apoptotic markers BcL-2, p21, and FasL also observed. These results suggest that SB contains phytochemicals that induce apoptosis in MDA-MB-157, 93A, and 93B by modulating these pro-apoptotic and anti-apoptotic proteins.
P-18 Chinese Medicinal herb Scutellaria barbata Modulates Apoptosis via Induction of Pro-apoptotic and Anti-apoptotic Proteins in MDA-MB-157, 93A, and 93B Breast Cancer Cells
Buller Hall Hallways
Scutellaria barbata (SB has been used in traditional Chinese medicine for treating liver, lung, and rectal cancers. It is included in most of the herbal cancer treatment formulas in Taiwan hospitals. We previously showed that aqueous extracts of SB inhibited mutagenesis, DNA binding, and metabolism of aflatoxin B1 and benzo(apyrene in carcinogenesis. A fraction of it was studied in a preliminary clinical trial in breast cancer patients. Breast cancer cell lines MDA-MB-157, 93A, and 93B are drug-resistant APC-mutants. In this study, the effectiveness of SB in the modulation of apoptosis of breast cancer cell lines MDA-MB-157, 93A, and 93B was investigated. Assessments were performed using green/red/blue fluorescent Apoptosis/Necrosis Detection Kit and the Human Apoptosis Antibody Array - Membrane (43 Targets test by the Abcam cooperation. Our data demonstrated that 1-hour treatment with 2 mg aqueous extract of SB induced a statistically significant percentage of apoptosis in MDA-MB- 157 (46.5 ± 7.5% > 16%± 1.0% , p < 0.05 ; 93A (54.5 ± 2.5% > 0%, p < 0.05; and 93 B (65.5 ± 13.5% > 0%, p < 0.05. Similar results were obtained with the 3-hour incubation period. Modulation of various apoptosis markers such as pro-apoptotic Bad, Bax, p53; and anti-apoptotic markers BcL-2, p21, and FasL also observed. These results suggest that SB contains phytochemicals that induce apoptosis in MDA-MB-157, 93A, and 93B by modulating these pro-apoptotic and anti-apoptotic proteins.