Date of Award
4-5-2019
Document Type
Project Report
Department
Chemistry & Biochemistry
First Advisor
Lisa Ahlberg
Abstract
HPLC methodology was developed to determine the concentrations and ratios of D to L amino acids in emu and ostrich eggshells treated with heat at different temperatures . We aimed to determine an internal standard and how ratios were affected under different conditions. An HPLC method was determined that gave consistent retention times and satisfactory enantioseparation . Calibration curves for each amino acid were developed from single and multi-amino acid containing dilution series producing a model that most closely replicates the eggshell-extracted amino acids.
Recommended Citation
Milkova, Gergana, "Analysis of DL-Amino Acid Ratios in Eggshells Using Reverse Phase-High Pressure Liquid Chromatography" (2019). Honors Theses. 205.
https://dx.doi.org/10.32597/honors/205
https://digitalcommons.andrews.edu/honors/205
Subject Area
High performance liquid chromatography; Eggshells, Amino acids
Creative Commons License
This work is licensed under a Creative Commons Attribution-No Derivative Works 4.0 International License.
DOI
https://dx.doi.org/10.32597/honors/205
Files over 3MB may be slow to open. For best results, right-click and select "save as..."