P-15 PAMAM Dendrimer Stability Analysis using Size-Exclusion Chromatography

Buller Hall 149

Enzymatic and thermal stability of dendritic nanoparticles is not well characterized in peer-reviewed literature. To establish methods and procedures to test nanoparticle stability, protocols were developed using bovine serum albumin (BSA) as a model for expensive PAMAM dendrimers since they share many of the same amide bond linkages. The amido structures was exposed to different environments such as pH, temperature, and proteases for various amounts of time and then evaluated using size exclusion chromatography (SEC). This research could prove beneficial to many disciplines that use dendrimer nanoparticles including medicinal uses involving oral delivery of pharmaceuticals.