Session

School of Population Health

Event Website

https://www.andrews.edu/services/research/research_events/conferences/urs_honors_poster_symposium/index.html

Start Date

3-26-2021 1:40 PM

End Date

3-26-2021 2:00 PM

Department

Population Health, Nutrition & Wellness

Description

Scutellaria barbata (SB) has been used in traditional Chinese medicine for treating liver, lung and rectal tumors. We previously found that aqueous extracts of SB inhibited mutagenesis, DNA binding, and metabolism of aflatoxin B1 and benzo(a)pyrene. They were also shown to inhibit foci formation in the colon of AOM-induced mice. Other research demonstrated that ethanol extracts inhibit colorectal cancer growth, inhibit proliferation and induce apoptosis. In this study, the effects of aqueous extracts of SB on the induction and modulation of apoptosis in HCT-116 colon cancer cells and CCD-841 normal colon epithelial cells were assessed using TUNEL assay, Apoptosis/Necrosis Detection Kit, and Human Apoptosis Antibody Array. Our findings suggest that SB possess a wide range of antitumor activity and may be a useful drug for treating colon cancer.

Acknowledgments

Advisor: Brian YY Wong

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Mar 26th, 1:40 PM Mar 26th, 2:00 PM

Selective induction of apoptosis by aqueous extract of Chinese medicinal herb Scutellaria barbata in HCT-116 colon cancer cells and CCD-841 colon epithelial cells.

Scutellaria barbata (SB) has been used in traditional Chinese medicine for treating liver, lung and rectal tumors. We previously found that aqueous extracts of SB inhibited mutagenesis, DNA binding, and metabolism of aflatoxin B1 and benzo(a)pyrene. They were also shown to inhibit foci formation in the colon of AOM-induced mice. Other research demonstrated that ethanol extracts inhibit colorectal cancer growth, inhibit proliferation and induce apoptosis. In this study, the effects of aqueous extracts of SB on the induction and modulation of apoptosis in HCT-116 colon cancer cells and CCD-841 normal colon epithelial cells were assessed using TUNEL assay, Apoptosis/Necrosis Detection Kit, and Human Apoptosis Antibody Array. Our findings suggest that SB possess a wide range of antitumor activity and may be a useful drug for treating colon cancer.

https://digitalcommons.andrews.edu/honors-undergraduate-poster-symposium/2021/symposium/36