Poster Title
P-24 Isolation and Characterization of Novel Arginine-Derived Heterocyclic Amines
Abstract
Most known heterocyclic amines (HCAs) are potent mutagens present at significant concentrations in cooked meats, and can be synthesized from burned mixtures of L-creatin(in)e with various amino acids. We hypothesize that novel mutagenic HCAs may be synthesized from a heated plant-based food simulating binary amino acid mixture: L-arginine and L-threonine. A two-part solid phase extraction is used to separate nitrogen heterocycles from byproducts followed by prep-HPLC to purify a small amount of a single compound, and characterization using GC-MS, IR, and NMR. The Ames mutagenicity assay using Salmonella TA98 is used to determine the mutagenic profile of the purified compound.
Location
Buller Hall Lobby
Start Date
3-8-2019 2:30 PM
P-24 Isolation and Characterization of Novel Arginine-Derived Heterocyclic Amines
Buller Hall Lobby
Most known heterocyclic amines (HCAs) are potent mutagens present at significant concentrations in cooked meats, and can be synthesized from burned mixtures of L-creatin(in)e with various amino acids. We hypothesize that novel mutagenic HCAs may be synthesized from a heated plant-based food simulating binary amino acid mixture: L-arginine and L-threonine. A two-part solid phase extraction is used to separate nitrogen heterocycles from byproducts followed by prep-HPLC to purify a small amount of a single compound, and characterization using GC-MS, IR, and NMR. The Ames mutagenicity assay using Salmonella TA98 is used to determine the mutagenic profile of the purified compound.
Acknowledgments
Supervising Professor: Dr. Ryan T. Hayes