Event Title

P-24 Isolation and Characterization of Novel Arginine-Derived Heterocyclic Amines

Location

Buller Hall Lobby

Start Date

3-8-2019 2:30 PM

Description

Most known heterocyclic amines (HCAs) are potent mutagens present at significant concentrations in cooked meats, and can be synthesized from burned mixtures of L-creatin(in)e with various amino acids. We hypothesize that novel mutagenic HCAs may be synthesized from a heated plant-based food simulating binary amino acid mixture: L-arginine and L-threonine. A two-part solid phase extraction is used to separate nitrogen heterocycles from byproducts followed by prep-HPLC to purify a small amount of a single compound, and characterization using GC-MS, IR, and NMR. The Ames mutagenicity assay using Salmonella TA98 is used to determine the mutagenic profile of the purified compound.

Acknowledgments

Supervising Professor: Dr. Ryan T. Hayes

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Mar 8th, 2:30 PM

P-24 Isolation and Characterization of Novel Arginine-Derived Heterocyclic Amines

Buller Hall Lobby

Most known heterocyclic amines (HCAs) are potent mutagens present at significant concentrations in cooked meats, and can be synthesized from burned mixtures of L-creatin(in)e with various amino acids. We hypothesize that novel mutagenic HCAs may be synthesized from a heated plant-based food simulating binary amino acid mixture: L-arginine and L-threonine. A two-part solid phase extraction is used to separate nitrogen heterocycles from byproducts followed by prep-HPLC to purify a small amount of a single compound, and characterization using GC-MS, IR, and NMR. The Ames mutagenicity assay using Salmonella TA98 is used to determine the mutagenic profile of the purified compound.