Event Title

Identification of the Eastern Massasauga Rattlesnake (Sistrurus catenatus) through Genetic Analysis of Shed Skin

Start Date

3-3-2017 2:30 PM

End Date

3-3-2017 4:00 PM

Description

The Eastern Massasauga Rattlesnake (Sistrurus catenatus) is considered an endangered species with only 45% of historic populations known to be extant today. This study aims to identify the presence of the Eastern Massasauga through genetic analysis and to determine genetic variability and diversity of populations in Michigan. Digestion of shed skins was performed by collagenase incubation followed by Proteinase K. Intronic regions of the TBP gene within the nuclear genome were amplified using PCR. A total of 7 out of 14 samples presented bands within expected product range (800pb). The purified PCR products will be sequenced and aligned with other sequences obtained from similar studies.

Acknowledgments

Dr. Peter J Lyons

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Mar 3rd, 2:30 PM Mar 3rd, 4:00 PM

Identification of the Eastern Massasauga Rattlesnake (Sistrurus catenatus) through Genetic Analysis of Shed Skin

The Eastern Massasauga Rattlesnake (Sistrurus catenatus) is considered an endangered species with only 45% of historic populations known to be extant today. This study aims to identify the presence of the Eastern Massasauga through genetic analysis and to determine genetic variability and diversity of populations in Michigan. Digestion of shed skins was performed by collagenase incubation followed by Proteinase K. Intronic regions of the TBP gene within the nuclear genome were amplified using PCR. A total of 7 out of 14 samples presented bands within expected product range (800pb). The purified PCR products will be sequenced and aligned with other sequences obtained from similar studies.