Event Title

Carboxypeptidase O Folding Requires a GPI Signal Peptide

Location

Buller Hall

Start Date

2-26-2016 2:30 PM

End Date

2-26-2016 4:00 PM

Description

Carboxypeptidase O (CPO) is a digestive enzyme that is thought to degrade proteins in the intestinal tract. Most related carboxypeptidases have a prodomain, which helps in protein folding. CPO in contrast, does not, but instead has a GPI membrane attachment, which may help in folding. Mutant CPO lacking the GPI anchor was poorly expressed in HEK293T and Sf9 cells when analyzed by western blotting. Experiments to determine the rate of degradation of CPO showed no difference between wild-type and mutant CPO. This suggests that the poor expression of mutant CPO is not due to the rate of degradation but possibly to the rate of production.

Acknowledgments

Dr. Peter J. Lyons

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Feb 26th, 2:30 PM Feb 26th, 4:00 PM

Carboxypeptidase O Folding Requires a GPI Signal Peptide

Buller Hall

Carboxypeptidase O (CPO) is a digestive enzyme that is thought to degrade proteins in the intestinal tract. Most related carboxypeptidases have a prodomain, which helps in protein folding. CPO in contrast, does not, but instead has a GPI membrane attachment, which may help in folding. Mutant CPO lacking the GPI anchor was poorly expressed in HEK293T and Sf9 cells when analyzed by western blotting. Experiments to determine the rate of degradation of CPO showed no difference between wild-type and mutant CPO. This suggests that the poor expression of mutant CPO is not due to the rate of degradation but possibly to the rate of production.