Presentation Title

P-27 The Correlation between the Milk Somatic Cell Count and the Differential Leukocyte Count in the Blood of Cows That Have a Positive Milk ELISA Test for Antibodies to the Bovine Leukosis Virus

Presenter Status

Professor of Animal Science, Department of Agriculture

Second Presenter Status

Associate Professor of Medical Laboratory Sciences, Department of Medical Laboratory Sciences

Third Presenter Status

Instructor in Medical Laboratory Sciences, Department of Medical Laboratory Sciences

Preferred Session

Poster Session

Start Date

26-10-2018 2:00 PM

End Date

26-10-2018 3:00 PM

Presentation Abstract

Bovine leukosis (BL) is a naturally-occurring transmissible retrovirus disease of cattle caused by the bovine leukosis virus (BLV) that is considered to be of economic importance in the international trade of animals and animal products. 89% of dairy herds in the U.S. have at least one infected animal. Presence of the virus is determined by measuring the BLV antibodies in a cow’s milk using an ELISA test. About 30-40% of cows that are infected show an increase in the quantity of white blood cells (WBCs) circulating in the bloodstream, however, the characterization of the types and ratios of WBCs has not yet been examined. It is hypothesized that some of these excess WBCs would end up in the udder since the lactating udder receives a very high blood flow, and this would therefore contribute to the elevated somatic cell count (SCC) in the milk. (For every liter of milk produced, 500 liters of blood circulate through the udder.) Therefore, a cow that is positive for BLV is likely to also have an elevated SCC in her milk.

Research Questions:

1) What is the correlation between the infection level by bovine leucosis virus and somatic cell count in cows’ milk?

2) What is the differential white blood cell count of the blood of cows that are negative for BLV, and those that test low-positive and high-positive for the virus?

3) What white blood cell type, if any, is more prevalent in BLV positive cows?

Acknowledgments

Additional budget allocation for Department of Medical Laboratory Sciences

Faculty Research Grant, Office of Research & Creative Scholarship, Andrews University

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Oct 26th, 2:00 PM Oct 26th, 3:00 PM

P-27 The Correlation between the Milk Somatic Cell Count and the Differential Leukocyte Count in the Blood of Cows That Have a Positive Milk ELISA Test for Antibodies to the Bovine Leukosis Virus

Bovine leukosis (BL) is a naturally-occurring transmissible retrovirus disease of cattle caused by the bovine leukosis virus (BLV) that is considered to be of economic importance in the international trade of animals and animal products. 89% of dairy herds in the U.S. have at least one infected animal. Presence of the virus is determined by measuring the BLV antibodies in a cow’s milk using an ELISA test. About 30-40% of cows that are infected show an increase in the quantity of white blood cells (WBCs) circulating in the bloodstream, however, the characterization of the types and ratios of WBCs has not yet been examined. It is hypothesized that some of these excess WBCs would end up in the udder since the lactating udder receives a very high blood flow, and this would therefore contribute to the elevated somatic cell count (SCC) in the milk. (For every liter of milk produced, 500 liters of blood circulate through the udder.) Therefore, a cow that is positive for BLV is likely to also have an elevated SCC in her milk.

Research Questions:

1) What is the correlation between the infection level by bovine leucosis virus and somatic cell count in cows’ milk?

2) What is the differential white blood cell count of the blood of cows that are negative for BLV, and those that test low-positive and high-positive for the virus?

3) What white blood cell type, if any, is more prevalent in BLV positive cows?