Presenter Status

Miss, Department of Agriculture and Industrial Technology

Presentation Type

Oral Presentation

Session

D

Location

Chan Shun 108

Start Date

19-5-2017 1:55 PM

End Date

19-5-2017 2:15 PM

Presentation Abstract

Biological Nitrogen Fixation (BNF) is one of the major sources of nitrogen to inherently low tropical soils (Bohool et al.,1992). The level of resistances of microorganisms to different concentrations of antibiotics is a factor for determining the degree of tolerance of microbes to stressful environments, especially microbes which take part in BNF. Carriers are also required for effective inoculant preparation to facilitate the survival and multiplication of rhizobium strains(microbes). Parent rhizobium strains USDA 3384 and USDA 3451 were exposed to antibiotics at different concentrations to determine their intrinsic resistance level. These were further seeded into three different solid carriers (peat, composted maize cob and cowdung). The developed mutant strains inoculated with the solid carriers and mutant strains inoculated without the solid carriers were evaluated in the laboratory and pot experiments and their effect with or without solid carriers on growth and dry matter yield of cowpea (Ife bimpe) were assayed. Data on plant growth and dry matter yield of cowpea were analysed using ANOVA and means were separated using Duncan Multiple Range Test (DMRT) at 5 % probability level.

USDA 3384 and USDA 3451 were resistant to Ampicillin trihydrate pensy at 500 mg/l and Streptomycin sulphate at 1000 mg/l respectively.

Biographical Sketch

Amarachi Grace Nwokocha started her training in 2002 at Michael Okpara University of Agriculture Umudike, Abia State Nigeria, where she obtained her Bachelors degree in Agriculture (Soil Science and Agroclimatology) in 2006/2007. After few years of service at Babcock University, She proceeded for her Masters degree in Soil Microbiology in 2013 at the University of Ibadan, and completed her program in 2015 with a result which placed her in a position to proceed straight for her PhD program. Currently She works as a Research Scientist in Babcock University Ilishan Remo Ogun State. She has contributed to knowledge through publications to reputable Journals with respect to sustainable agriculture in sub-sahara Africa and the likes.

Included in

Agriculture Commons

COinS
 
May 19th, 1:55 PM May 19th, 2:15 PM

Resistance of Two B. Japonicum Strains to Antibiotics and Their Effect with or without Solid Carriers on Growth and Biomass Yield of Cowpea

Chan Shun 108

Biological Nitrogen Fixation (BNF) is one of the major sources of nitrogen to inherently low tropical soils (Bohool et al.,1992). The level of resistances of microorganisms to different concentrations of antibiotics is a factor for determining the degree of tolerance of microbes to stressful environments, especially microbes which take part in BNF. Carriers are also required for effective inoculant preparation to facilitate the survival and multiplication of rhizobium strains(microbes). Parent rhizobium strains USDA 3384 and USDA 3451 were exposed to antibiotics at different concentrations to determine their intrinsic resistance level. These were further seeded into three different solid carriers (peat, composted maize cob and cowdung). The developed mutant strains inoculated with the solid carriers and mutant strains inoculated without the solid carriers were evaluated in the laboratory and pot experiments and their effect with or without solid carriers on growth and dry matter yield of cowpea (Ife bimpe) were assayed. Data on plant growth and dry matter yield of cowpea were analysed using ANOVA and means were separated using Duncan Multiple Range Test (DMRT) at 5 % probability level.

USDA 3384 and USDA 3451 were resistant to Ampicillin trihydrate pensy at 500 mg/l and Streptomycin sulphate at 1000 mg/l respectively.