Date of Award
4-12-2024
Document Type
Honors Thesis
Department
Chemistry & Biochemistry
First Advisor
Lisa Ahlberg
Abstract
The goal of this research was to determine the mechanism behind 1,1,1,3,3,3-hexafluoroisopropanol's (HFIP0 ability to suppress the separation of oligonucleotide diastereomers. This was accomplished by selecting different molecules that were like HFIP but differed in specific ways such as polarity, number and type of halogen(s), alcohol group location, carbon chain length, hydrophobicity, and chao/kosmotropicity. These molecules replaced HFIP in an IPRP chromatography method to determine if splitting of the diastereomers was observed and then they would becompared to HFIP's effect. Viscosity tests were to run to acquire quantitative data to determine chao/kosmotropicity. No decisive trend among a single factor was observed meaning that more than one factor effects diastereomer selectivity.
Note that this research is qualitative in anture and has no statistical analysis.
Recommended Citation
Neidigh, Ian, "Understanding the Mechanism Behind HFIP's Effect on Diastereomeric Separation of Oligonucleotides" (2024). Honors Theses. 286.
https://digitalcommons.andrews.edu/honors/286
Subject Area
Diastereoisomers, Oligonucleotides
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.