Date of Award
4-5-2019
Document Type
Honors Thesis
Department
Chemistry and Biochemistry
First Advisor
Ryan T. Hayes
Abstract
Most known heterocyclic amines (HCAs) are potent mutagens present at significant concentrations in cooked meats, and can be synthesized from burned mixtures of L-creatin(in)e with various amino acids. We hypothesize that novel mutagenic HCAs may be synthesized from a heated plant-based food simulating binary amino acid mL'\:ture: L-arginine and L-threonine. A two-part solid phase extraction was used to separate nitrogen heterocycles from byproducts followed by prep HPLC and the Ames mutagenicity assay using Salmonella TA98 to determine the mutagenicity of various isolates. The results indicate that this binary amino acid mixture does not yield a mutagenic sample.
Recommended Citation
Srikureja, Nathaniel, "Isolation and Characterization of Novel Arginine-Derived Heterocyclic Amines" (2019). Honors Theses. 213.
https://dx.doi.org/10.32597/honors/213
https://digitalcommons.andrews.edu/honors/213
Subject Area
Heterocyclic compounds; Amines;
Creative Commons License
This work is licensed under a Creative Commons Attribution-No Derivative Works 4.0 International License.
DOI
https://dx.doi.org/10.32597/honors/213
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