Event Title

Analysis of Mammalian Carboxypeptidase O Expression Patterns

Location

Buller Hall

Start Date

2-26-2016 2:30 PM

End Date

2-26-2016 4:00 PM

Description

Carboxypeptidase O (CPO) is a protease that cuts acidic amino acids from the carboxy termini of substrate proteins. This study aims to describe the expression patterns of CPO in small mammals. We have sampled tissue from four different species: thirteen-lined ground squirrel, eastern chipmunk, eastern mole, and northern short-tailed shrew. Samples were analyzed via Western blot using two CPO antibodies. Immunoreactive bands likely to be CPO, with molecular weights approximately 42 kDa, were seen predominantly in kidney, liver, and small intestine tissue samples. This suggests CPO could play a specific physiological function in these organs.

Acknowledgments

Dr. Peter J. Lyons

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Feb 26th, 2:30 PM Feb 26th, 4:00 PM

Analysis of Mammalian Carboxypeptidase O Expression Patterns

Buller Hall

Carboxypeptidase O (CPO) is a protease that cuts acidic amino acids from the carboxy termini of substrate proteins. This study aims to describe the expression patterns of CPO in small mammals. We have sampled tissue from four different species: thirteen-lined ground squirrel, eastern chipmunk, eastern mole, and northern short-tailed shrew. Samples were analyzed via Western blot using two CPO antibodies. Immunoreactive bands likely to be CPO, with molecular weights approximately 42 kDa, were seen predominantly in kidney, liver, and small intestine tissue samples. This suggests CPO could play a specific physiological function in these organs.